KMID : 0900919980220010089
|
|
Korean journal of Animal Reproduction 1998 Volume.22 No. 1 p.89 ~ p.94
|
|
Recovery of IGF-I Using Liquid Emulsion Membranes
|
|
|
|
Abstract
|
|
|
A study was made to investigate the effects of concerning factors with IGF-I recovery on the final IGF-I concentration in the effluent and to establish recovery conditions of IGF-I using liquid emulsion membranes(LEM). D2EHPA was best carrier among Amberlite LA2, Aliquit 336 and D2EHPA for recovery rate of IGF-I. Recovery rate of IGF-I by D2EHPA volume in the oil phase was increased as increasing D2EHPA volume, and optimal volume of D2EHPA was 5% in this experiment. The recovery rate of IGF-I by D2EHPA was increased by the decreasing from pH 7 to pH 4 of external phase. Therefore, optimal pH value was 4.0. Optimal concentrations of sulfuric acid in internal phase, paraffin oil in oil phase and Span 80 for recovery rate of IGF-I were 0.1M, 2.0% and 5%, respectively, and optimal W/O rate was 2. These results suggested that optimal conditions for recovery of IGF-I were D2EHPA(5%) as carrier, pH 4.0, 0.1M sulfuric acid, 2% paraffin oil, 2.0 W/O rate and 5.0% Span 80.
|
|
KEYWORD
|
|
IGF-I, Liquid emulsion membranes, D_2EHPA, Sulfuric acid, Paraffin oil, W/O rate, Span 80
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|